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Macromolecules are the proteins and nucleic acids upon which life depends. Understanding the action of biological macromolecules (giant molecules) requires detailed knowledge of their structures. Most of the more than ten thousand known structures of protein and nucleic acids were obtained by x-ray crystallography, the standard mechanism for determining protein structure. Essentially, proteins are frozen into rigid crystals, which can be stacked up in a repeating pattern--like supermarket displays. The structure of each individual crystal can be determined by the way x-rays are bent when they pass through the composite crystal. Protein structure is essential when investigating protein interactions and planning drug development.
Crystallography Made Crystal Clear, Second Edition explains how scientists discover the structures of the macromolecules. Scientists do not see these molecules directly. Instead, they build models as a means of interpreting data from x-ray diffraction by crystals, or by irradiation by other forms of energy. Users of these models need to know how they are obtained in order to know what they are seeing when they study a model of a macromolecule. They also need to know how to judge whether conclusions they draw from the molecular models are really supported by the models. This book uses visual and geometric models to help readers understand the mathematics that forms the basis of x-ray crystallography.
The field of protein crystallography is growing every day and has been instrumental in discovering the molecular principles of biology and in discovering new drugs, such as the recent protease inhibitors for AIDS. The field includes the largest percentage of Nobel prizes than any other scientific discipline. Every major university and drug company has a protein crystallography laboratory and this book is an invaluable aid to those wishing to practice protein crystallography
or just learn more about how it is actually done.
Key Features
* Provides clear, understandable descriptions of principles of X-ray crystallography
* Leads reader through unintimidating and thorough explanations of the underlying mathematics
* Provides abundant illustrations, including diagrams, charts, photographs, and color stereo
* images
* Explains how to read crystallography papers in research journals
* Includes brief descriptions of other diffraction methods (neutron, electron, Laue) and the kinds
* of structural information they can provide
* Introduces other methods of macromolecular structure determination (NMR spectroscopy and
* homology modeling), and provides guidance in judging the quality of these models
- Sales Rank: #2160942 in Books
- Published on: 1999-12-13
- Original language: English
- Number of items: 1
- Dimensions: .56" h x 5.97" w x 8.97" l,
- Binding: Paperback
- 286 pages
Review
"... I would recommend this book to anyone who is interested in macromolecules and how their structures are solved. The material is well presented and easy to read and would provide a good starting point for an undergraduate considering going into the field. It also provides sufficient information to be used as a text in a course on biophysical techniques." -- Biochemical Education
"Crystallography Made Crystal Clear bridges the gap between brief chapters and textbooks in biochemistry and proteins and complete treatments aimed at the professional crystallographer...All of the problems one encounters, from recognizing twinned crystals and visualizing the geometry of a precession camera, through identifying heavy atom binding sites from Patterson maps, to fitting electron density maps and refining the structure are dealt with patiently and creatively...the text has a light touch which both novices and noncrystallographers will appreciate...The thirteen color plates are excellent...Given the brevity of the text, it is remarkably complete...This book will be useful in many contexts - in elementary courses in crystallography, in biochemistry courses as an auxiliary text, in crystallographic laboratories as a handbook for novices, and in molecular biology laboratories as an introduction to the Protein Data Base and molecular graphics. It can be perused in an afternoon which will be well spent." -- Biophysical Journal
"Anyone interested in how protein structures are determined should find reading it an enjoyable and satisfying experience...Crystallography Made Crystal Clear is clearly written, accurate, and easy to read. The author Chose one of the most interesting topics in x-ray crystallography to examine, namely, the structure determination of proteins. Consequently the book can be recommended not only to the biochemists and biologists for whom it was written, but to all those who are curious." -- Applied Optics
"Crystallography is not an easy subject to teach or to learn, and Rhodes provides a comprehensive, yet less intimidating, treatment of the theoretical background, which should be understandable to a novice. The author assumes little mathematical knowledge and explains the physical significance of all equations. A most helpful feature is the use of a published structure report as an example of understanding and interpreting a macromolecular crystal structure determination, frequently the most difficult part for noncrystallographers. Highly recommended as a supplement to standard biochemistry works and as an introduction to the field for students learning crystallography." -- Choice
"This terse, well-written book lives up to its title in great measure, and, in my opinion is now the best reference for noncrystallographers who want to know more about X-ray diffraction and the data that result from it." -- American Journal of Physiology
"[A] successful introduction for those who try to understand and explore biological macromolecular structures....The text is loaded with many excellent didactic concepts and approaches." -- ACTA Cryst
Praise for the First Edition "Had [this book] appeared ten years ago, learning the tools of this trade would have been easier." -- Trends in Biotechnology
From the Back Cover
Gale Rhodes makes crystallography accessible to readers who have no prior knowledge of the field, or its mathematical basis. The second edition of Crystallography Made Crystal Clear has been fully updated and expanded to make it the most comprehensive and concise reference for beginning crystallographers. The book also introduces essential World Wide Web tools for users of models, including beginning-level tutorials in molecular modeling on personal computers. The CMCC Home Page (www.usm.maine.edu/~rhodes/CMCC) provides access to all tools and links to all resources discussed in this book. Most significantly, the final chapter introduces the reader to macromolecular modeling on personal computers-featuring SwissPdbViewer, a free, powerful modeling program now available for PC, Power Macintosh, and Unix computers.
Key Features
* Provides clear, understandable descriptions of principles of X-ray crystallography
* Leads reader through unintimidating and thorough explanations of the underlying mathematics
* Provides abundant illustrations, including diagrams, charts, photographs, and color stereo
* images
* Explains how to read crystallography papers in research journals
* Includes brief descriptions of other diffraction methods (neutron, electron, Laue) and the kinds
* of structural information they can provide
* Introduces other methods of macromolecular structure determination (NMR spectroscopy and
* homology modeling), and provides guidance in judging the quality of these models
About the Author
Gale Rhodes earned a B.S. in applied mathematics at North Carolina State University, and then a Ph.D. in Chemistry at the University of North Carolina. He is currently a professor of chemistry at the University of Southern Maine, Portland. His main duty, and first love, is teaching undergraduate biochemistry. He has received awards for outstanding teaching at three different colleges. His best known publication is the first edition of Crystallography Made Crystal Clear, which received very complimentary reviews in several journals. He has also published three book chapters, three book reviews, and about 30 articles on diverse subjects, including research articles in biochemistry, and articles on chemistry, science, and interdisciplinary education.
Most helpful customer reviews
0 of 0 people found the following review helpful.
An appropriate book.
By irene ogutu
this book will be of great use to me as a beginner in crystallography and help me with my studies.
5 of 5 people found the following review helpful.
Buy "Biomolecular Crystallography"
By Tommy Carstensen
Buy "Biomolecular Crystallography" instead. This book tries to condense too much information on too few pages. I didn't understand everything in "Biomolecular Crystallography", but the sections that I did understand, are very well written. It's more expensive, but it's a better book, for those that really want to understand crystallography beyond various tutorials on the internet and "Crystallography Made Crystal Clear".
6 of 7 people found the following review helpful.
Clear perhaps, accurate not
By Dr. Peter Muller
While Crystallography made Crystal Clear by Gale Rhodes is certainly easy to read, it oversimplifies things and introduces a few inaccuracies. It falls short to make the student really understand crystallography, which, in my opinion, is not a good thing for a textbook. On the other hand, I have made the experience that, unfortunately, many students are happy with only a superficial knowledge of the basic theory (I have been teaching crystallography for about a decade now) and this book helps with this attitude.
My advice: if you just want to pass the class and don't care about the subject, go with this book. If, however, you really want to understand what crystallography is about, you need to read either the great beginner's book by Werner Massa: Crystal Structure Determination or the more protein crystallography oriented but equally great beginners book by Jenny Glusker and Kenneth Trueblood: Crystal Structure Analysis -- A Primer. Once you have digested the Massa or the Glusker/Trueblood, you can work your way through the book edited by Carmelo Giacovazzo: Fundamentals of Crystallography.
After reading and understanding the Massa or Glusker/Trueblood and the Giacovazzo, you'll be ready to survive a discussion with any crystallographer any time. (What is more: you will enjoy the discussion!) The book by Gale Rhodes, however, will only get you over the exam and you'll miss out on the great fun crystallography can be.
EDIT on 12/12/2010:
A new edition of the Glusker Trueblood has come out: Crystal Structure Analysis: A Primer. The classic has been brought up-to-date and there really is no excuse any more for reading any other introductory textbook than the Glusker/Trueblood. Especially not the book by Gale Rhodes.
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